Fluxion Biosciences - Applications (Cellular Biology, Microbiology, Ephys)

Applications

Automated Electrophysiology

Fluxion's IonFlux system provides automated patch clamping for recording from ligand-gated and voltage-gated ion channels. A wide variety of electrophysiology applications are supported, including ion channel drug screening and pharmacology.

For more information on the IonFlux system, please click here.

For more information on IonFlux applications, please visit our Library section for additional downloads.

Representative Examples of Automated Electrophysiology Experiments Using IonFlux
	GABA-A channel recording Continuous recording from an ensemble of CHO cells expressing GABA-A receptors during successive application of increasing GABA concentrations is shown. The reported EC₅₀ is 3µM.
	Kv2.1 Currents During Perfusion Responses to two TEA (tetraethylammonium) concentrations are shown in two different cells. Voltage step protocol eliciting a full I-V curve was performed during steady state TEA perfusion. 15-30% inhibition was observed at 10mM TEA, while almost complete inhibition was observed at 100mM TEA. Post-perfusion measurements reflect slow inactivation of the Kv2.1 channel over time.
	P2X3 Agonist evoked inward currents from two different RLE cells during exposure to αβ methylene(Me) ATP in RLE cells stably transfected with hP2X3 receptor/channels.
	TRPV1 Membrane current recorded in response to 50µM capsaicin applied for 5s to a single CHO – hTRPV1 cell at one trapping site in an IonFlux 100 device. Normalized dose-response curve based on recordings similar to the left panel.