Cellular Monolayers

Cellular monolayers are frequently grown and studied as a way of observing cells in a more physiologically-relevant context. Many cell adhesion or host-pathogen interaction studies require a monolayer of cells as the adhesion substrate. The BioFlux System provides an ideal platform for monolayer growth and analysis. The BioFlux Plate microfluidic channels feature coverslip glass bottom surfaces (180µm) which facilitate high resolution imaging. The channels themselves can be coated with a variety of natural and artificial growth matricies, such as:

• Collagen
• Fibronectin
• Poly-L-lysine
• Matrigel
• Gelatin
• Laminan

An endothelial cell monolayer cultured in a BioFlux channel using gelatin as an adhesion promoter.

More information can be found in our Cellular Biology Application Notes