BioFlux System
Product Information
(14 items)Pulsatile Flow Module - Technical Note (Adobe PDF, 177 KBytes)
BioFlux Prep Station Product Data Sheet (Adobe PDF, 135 KBytes)
BioFlux 1000Z Product Data Sheet (Adobe PDF, 290 KBytes)
BioFlux Montage Brochure (Adobe PDF, 537 KBytes)
Provides a detailed overview of the BioFlux Montage Software Package which works with the BioFlux 1000 system.
BioFlux Grant and Publication Kit (Zip Archive, 33.7 MBytes)
Contains useful figures, product photos, well plate diagrams, and written descriptions for the BioFlux System. Appropriate for new grant applications, scientific publications, and budget justifications.
BioFlux Ordering Information (Adobe PDF, 22 KBytes)
BioFlux Product Brochure (Adobe PDF, 1.7 MBytes)
High Shear BioFlux Plate (Adobe PDF, 444 KBytes)
Data sheet for High Shear BioFlux Plate. Designed to reach higher shear rates for vascular, platelet, thrombosis, and adhesion assays.
BioFlux 1000 Product Data Sheet (Adobe PDF, 263 KBytes)
Contains a product overview and description along with key performance specifications.
BioFlux 200 Product Tutorial (Adobe PDF, 1.7 MBytes)
An illustrated overview of BioFlux principals, components and applications.
BioFlux Technology White Paper (Adobe PDF, 3.2 MBytes)
Provides a more detailed description of BioFlux technology and principles of operation.
BioFlux 200 Product Data Sheet (Adobe PDF, 276 KBytes)
Contains a product overview and description along with key performance specifications.
Publications
(17 items)Mol Microbiology (Donohue) - candida albicans (Adobe PDF, 52 KBytes)
Cardiovasc Res (James) - shear stress on endothelial cells (Adobe PDF, 68 KBytes)
JALA (Conant) - shear based thrombosis (Adobe PDF, 51 KBytes)
Biotech Bioeng (Nevill) - dynamic platelet behavior (Adobe PDF, 67 KBytes)
J Translational Med (Pandey) - platelet aggregation (Adobe PDF, 75 KBytes)
Blood (Martin) - Thrombus formation in vitro and in vivo (Adobe PDF, 16 KBytes)
Helicobacter pylori AlpA and AlpB bind host laminin (Adobe PDF, 33 KBytes)
Published in Infection and Immunity, 2011
New device for high-throughput viability screening of flow biofilms (Stanford University - Matin Lab) (Adobe PDF, 25 KBytes)
Appl Environ Microbiol. 2010 Jul;76(13):4136-42. Epub 2010 Apr 30.
PMID: 20435763 [PubMed - in process]
Shear-Enhanced Oral Microbial Adhesion (NIH - Palmer Lab) (Adobe PDF, 24 KBytes)
Publication in J. of Applied and Environmental Microbiology from researchers at the NIH.
Platelet adhesion and aggregation under flow using microfluidic flow cells (Adobe PDF, 24 KBytes)
Journal of Visualized Experiments (Oct 2009)
Wound Healing Assays in Well Plate–Coupled Microfluidic Devices with Controlled Parallel Flow (JALA - Conant) (Adobe PDF, 56 KBytes)
Journal of the Association for Laboratory Automation (Feb 2010)
Well Plate-Coupled Microfluidic Devices Designed for Facile Image-Based Cell Adhesion and Transmigration Assays (JBS - Conant) (Adobe PDF, 24 KBytes)
Journal of Biomolecular Sciences - Dec 2009
How It Works Article - Lab Manager (Adobe PDF, 92 KBytes)
Article published in Lab Manager Magazine describing utility of BioFlux system for solving critical problems in life science research and drug discovery.
Differentiation of Rat Mesenchymal Stem Cells Under Shear Stress (Millipore Cellutions) (Adobe PDF, 740 KBytes)
Stem cell research has the potential to produce novel treatments for previously incurable diseases and injuries. For
studies of stem cell differentiation, scientists have traditionally used biochemical stimulation. However, shear
stress can also be used. Here we demonstrate the differentiation of rat mesenchymal stem cells into von Willebrand factor (vWF)-expressing endothelial cells as a result of shear stress.
Shear Flow Bibliography (Adobe PDF, 117 KBytes)
A list of rlevant articles from the literature which highlight the effects and importance of shear flow in biological systems.
Live Cell Imaging Advances (Drug Discovery & Development) (Adobe PDF, 57 KBytes)
A review of current approaches to live cell imaging featured in Drug Discovery & Development (Feb 2008).
High Throughput Live Cell Imaging Assays (Gen Engineering News)) (Adobe PDF, 55 KBytes)
An overview article describing live cell assays for microbiology and cellular adhesion using the BioFlux 200. Presents an application overview and outlines some of the key benefits to running these assays using Well Plate Microfluidics.
Application Notes - Cell Biology
(12 items)Cellular Migration and Invasion (Adobe PDF, 733 KBytes)
Application note covering BioFlux's unique ability to create microenvironmental regions using parallel flow, and utilize real-time imaging for kinetic observation.
Angiogenesis (Adobe PDF, 583 KBytes)
Cancer cell invasion assay with real time imaging.
Neurite Outgrowth (Adobe PDF, 2.4 MBytes)
This application note describes a neurite outgrowth assay using the BioFlux system. Illustrates how the fluid addition steps can be done utilizing the rapid fluid exchange capabilties, without harming the neurite monolayer.
Stem Cell Differentiation (Adobe PDF, 2.9 MBytes)
This application note describes use of the BioFlux System for running stem cell differentiation experiments. Highlights an example which depicts mesenchymal stem cells differentiated into endothelial cells utilizing shear stress.
Wound Healing (Adobe PDF, 1.1 MBytes)
Transmigration (Adobe PDF, 711 KBytes)
Stem Cells (Adobe PDF, 1.7 MBytes)
Rolling Adhesion (Adobe PDF, 2.3 MBytes)
Platelet Adhesion (Adobe PDF, 663 KBytes)
High Throughput Cell Adhesion (Adobe PDF, 1.4 MBytes)
Endothelial Cell Adhesion (Adobe PDF, 262 KBytes)
Cellular Adhesion Inhibition (Adobe PDF, 235 KBytes)
Application Notes - Microbiology
(5 items)High Content Analysis for Biofilms (Adobe PDF, 1.6 MBytes)
Anaerobic Biofilms (Adobe PDF, 1.3 MBytes)
Host Pathogen Interactions (Adobe PDF, 1.9 MBytes)
Biofilm Growth (Adobe PDF, 316 KBytes)
Biofilms Compound Screening (Adobe PDF, 506 KBytes)
Technical Notes
(12 items)BioFlux PC Requirements (Adobe PDF, 222 KBytes)
Provides the minimum and recommended computer requirements to run BioFlux software.
Whole Blood Assays (Adobe PDF, 4.2 MBytes)
Describes best practices for running whole blood assays on the BioFlux System. Includes cleaning procedures.
High Content Screening Kits (Adobe PDF, 3.4 MBytes)
This technical note describes the use of commercially-available fluorescence based staining kits for high content analysis. Highlights an example of using toxicology markers for epithelial cells under shear flow.
Viscosity in the BioFlux System (Adobe PDF, 1.2 MBytes)
Provides a general overview of fluid viscosity and how it relates to BioFlux experiments. Includes suggestions for how to estimate and measure viscosity of a variety of biological fluids, including whole blood.
Imaging Capabilities (Adobe PDF, 4.3 MBytes)
An overview of BioFlux imaging properties and description of common imaging modalities including brightfield, phase contrast, fluorescence and confocal microscopy. Includes sample images.
Preconditioning Endothelial Cell Monolayers (Adobe PDF, 1.8 MBytes)
Growing Cellular Monolayers (Adobe PDF, 1.8 MBytes)
Growing Airway Epithelial Cell Monolayers (Adobe PDF, 1.3 MBytes)
Reproducibility of Cellular Adhesion Assays (Adobe PDF, 765 KBytes)
Protocols
(4 items)Platelet Protocol - Physiological Shear (Adobe PDF, 2.6 MBytes)
Platelet Protocol - High Shear (Adobe PDF, 3.7 MBytes)
Platelet Inhibition Protocol (Adobe PDF, 2.5 MBytes)
Micropatterning Matrigel in the BioFlux System (Adobe PDF, 64 KBytes)
Protocol for the angiogenesis and cell invasion assay.
Poster Presentations
(15 items)ASCB 2010 Poster - Pulsatile Flow (Adobe PDF, 1.2 MBytes)
ASCB 2010 Poster - Wound Healing (Adobe PDF, 881 KBytes)
Cancer Cell Invasion Poster Presentation (Adobe PDF, 1.1 MBytes)
Poster presentation from AACR 2010
Angiogenesis Poster Presentation (Adobe PDF, 1.1 MBytes)
Poster presentation from SBS 2010. Covers angiogenesis and cancer cell invasion applications.
ASCB 2009 Poster - Angiongenesis and Cell Invasion (Adobe PDF, 1.3 MBytes)
We demonstrate a microfluidic based method to follow angiogenesis or cell invasion in real time. The method enables acquisition of high content data by microscopy using microfluidic flow cells which are optimized for imaging.
THE INHIBITION OF TROPHOBLAST APOPTOSIS (St Georges Univ of London - James) (Adobe PDF, 132 KBytes)
JL James, GStJ Whitley, JE Cartwright.
St George's, University of London.
Poster presentation from the International Society for the Study of Hypertension in
Pregnancy.
A Microfluidic System Designed to Observe Platelet Behavior under Controlled Shear in Real Time (ISTH 2009) (Adobe PDF, 950 KBytes)
Poster presentation covering thrombosis and platelet adhesion applications.
BioRheology 2008 Poster (Adobe PDF, 1.2 MBytes)
Covers use of BioFlux system for cancer (colon carcinoma) cell adhesion applications.
Experimental Biology 2008 Poster (Adobe PDF, 910 KBytes)
Covers use of BioFlux system for rolling adhesion and firm cell adhesion.
Lab Automation 2009 Poster (Adobe PDF, 943 KBytes)
Covers use of BioFlux system for cell adhesion applications.
CHI Cell Based Assays for HTS 2008 Presentation (Adobe PDF, 947 KBytes)
PowerPoint presentation covering use of BioFlux system for cell-based assays under shear flow.
CHI High Content Analysis 2009 Poster (Adobe PDF, 2.6 MBytes)
Covers use of BioFlux system for cell adhesion and high content analysis assays using the CellCiphr toxicology kit.
BioFilms III 2008 Poster (Adobe PDF, 551 KBytes)
Covers use of BioFlux system for growing and analyzing biofilms under shear flow.
American Society Matrix Biology 2008 Poster (Adobe PDF, 1.2 MBytes)
Covers use of BioFlux system for cell adhesion, transmigration and wound healing.
American Society of Cell Biology 2008 Poster (Adobe PDF, 1.2 MBytes)
Covers use of BioFlux system for cell adhesion, transmigration and wound healing.