IonFlux System
| Product Information (4 items) |  |
 IonFlux Product Data Sheet (PDF, 0.3MB) - Brief Product Overview IonFlux Product Brochure (PDF, 1.3 MB) - Introduction to the IonFlux Family IonFlux F1 Giga-Ohm Seal Plates (PDF, 0.2MB) - Single Cell G-seal Assay Performance IonFlux Well Plate Layout (PDF, 0.3MB) - The Microfluidic Layout of 96- and 384-well IonFlux Plates
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| Application Notes & Assay Data (15 items) | |
Validated Cell Lines and Ion Channels on IonFlux (PDF, 0.2MB) - A comprehensive list of cell lines and ion channel targets tested and validated on the IonFlux SystemLigand-Gated Channels GABA-A Application Note (PDF, 0.6MB) - An overview of GABA-A application data including success rates, assay repeatability, and modulator pharmacology. Screening of GABA-A Receptor Subtypes Using Transient Transfection (PDF, 0.2MB) - Workflow development for successful characterization of a large amount of GABA-A subunit compositions, (Customer Presentation, Alexander Arnold, PhD) Endogenous GABA-A Channels (PDF, 0.5MB) - Feasibility data for recording from ex vivo immortalized smooth muscle cells Customer Data, Charles Emala, PhD) NMDA Receptor Validation Data (PDF, 0.3MB) - Continuous perfusion and synchronous compound addition enable a high success rate assay for a challenging ion channel target. Nicotinic Acetylcholine Receptors Application Note (PDF, 0.8MB) - Validation of naChR assays. An Automated Electrophysiological Assay for the a4b2 Nicotonic Receptor (PDF, 13.5MB) - Assay success rates, EC50 repeatability, experiment run times and pharmacology (Customer Presentation, Elaine Gay, PhD) Transiently Transfected Cell Lines for GABA Receptor Screening using the IonFlux System (PDF, 0.2MB) - Application NoteVoltage Gated Channels hERG Safety Pharmacology (PDF, 0.8MB) - Biophysical characterization of hERG currents and the pharmacological inhibition profiles of a panel of known blockers. B'SYS CHO hERG Characterization & Functional Validation with IonFlux Single Mode HT (PDF, 3.9MB) A head to head comparison of the IonFlux hERG pharmacology to existing PatchX and manual patch clamp at Sanofi (PDF, 0.6MB) - Optimization of hERG screening using IonFlux HT automated system, Sanofi, conference poster presentation, 2011. Temperature Dependent hERG Pharmacology (PDF, 0.3MB) - A comparison of room temperature vs. physiological temperature hERG channel kinetics and pharmacology. Nav Assay Characterizationv(PDF, 0.3MB) - Overview of Nav1.7 and Nav1.8 current recordings and pharmacology of lidocaine. Nav - Use Dependence Assays (PP, 2.2MB) - Recovery from inactivation and use dependent pharmacology for a Nav1.7/1.8 blockers (slide presentation) Kir - Development of an HTS Followup Screen (PDF, 12 MB) - See how an Ionflux customer developed an automated patch clamp assay for Kir 4.1 (Customer presentation, Jarod Denton, PhD) Genedata Screener Software for Ion Channels (PDF, 2.0MB) - This module enables plate data management, fitting and compound potency databasing for large IonFlux data sets. |
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| Publications & Posters (5 items) | |
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Validation of a Set Ion Channel Assays Using Continuous Perfusion in Collaboration with Pfizer, KV2.1, Nav1.7-1.8, GABA, and nAChR (PDF, 0.7MB) - Ion Channel Pharmacology Under Flow: Automation Via Well Plate Microfluidics, Assay and Drug Development Technologies 2012. Development of Transient Transfection and Endogenous Expression Systems for LGIC Assays (PDF, 0.7MB) - Comparison of Cell Expression Formats for the Characterization of GABAA Channels Using a Microfluidic Patch Clamp System, Assay and Drug Development Technologies, 2012. HERG Pharmacology and Temperature Dependence at Novartis (PDF, 0.8MB) - IonFlux: A Microfluidic Patch Clamp System Evaluated with Human Ether-a-go-go Related Gene Channel Physiology and Pharmacology, Assay and Drug Development Technologies, 2011. A head to head comparison of the IonFlux hERG pharmacology to existing PatchX and manual patch clamp at Sanofi (PDF, 0.6MB) - Optimization of hERG screening using IonFlux HT automated system, Sanofi, conference poster presentation, 2011. A First Publication of Feasibility Data for the IonFlux Microfluidic Patch Technology (PDF, 0.5MB) - Mammalian electrophysiology on a microfluidic platform, PNAS, 2005 |
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| Presentations (6 items) | |
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A GABA-A Ligand Gated Ion Channel Screen - Results and Best Practices (PDF, 0.9MB) - Biophysics 2011 (poster presentation) A Nicotonic Receptor Assay Showing Fast Recovery Time Using Continuous Perfusion (PDF, 0.9MB) Biophysics 2011 (poster presentation) Temperature Dependence on hERG Blocker Pharmacology (PDF, 0.5MB) - Biophysics 2011 (poster presentation) Screening of GABA-A Receptor Subtypes Using Transient Transfection (PDF, 0.2MB) - Workflow development for successful characterization of a large amount of GABA-A subunit compositions, (Customer Presentation, Alexander Arnold, PhD) Endogenous GABA-A Channels (PDF, 0.5MB) - Feasibility data for recording from ex vivo immortalized smooth muscle cells (Customer Data, Charles Emala, PhD) Kir - Development of an HTS Followup Screen (PDF, 12 MB) - See how an Ionflux customer developed an automated patch clamp assay for Kir 4.1 (Customer presentation, Jarod Denton, PhD) Giga-seal Single Cell Recording as Compared to Ensemble Recording (PDF, 3.3MB) |
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| Webinars (8 items) | |
 Enabling a High Efficiency Assay for GABA Isoforms using Transient Transfection (45 min.) (Leggy Arnold, University of Wisconsin, Milwaukee) Development of a High Throughput IonFlux Assay to Detect Positive Allosteric Modulators of the GABA-A Receptor (45 min.) Juha Kammonen, Pfizer - Neusentis hERG Assay Development on the IonFlux System Using Gigaseal & Ensemble Recordings (30 min.) Ali Yehia, IonFlux Sales Director, Fluxion Biosciences IonFlux - Simplified Pharmacology (30 min.) Steve Smith, IonFlux Technical Director, Fluxion Biosciences IonFlux - APC Without Compromises (40 min.) Cristian Ionescu - Zanetti, CTO, Fluxion Biosciences IonFlux: Automated Electrophysiology with Plate Reader Simplicity (60 min.) Steve Smith, IonFlux Technical Director, Fluxion Biosciences Automated Patch Clamp for the Research Environment (20 min.) Steve Smith, IonFlux Technical Director, Fluxion Biosciences Automated Patch Clamp With Temperature Control (15 min.) Cristian Ionescu - Zanetti, CTO, Fluxion Biosciences |
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