Fluxion Biosciences

The CytoFlux imaging cytometer combines the statistical power and throughput of flow cytometry with the information-rich imaging of high resolution microscopy. The availability of individual cell images creates information-rich data on intracellular organization and cell morphology, thus enabling new applications that rely on intracellular imaging.

The CytoFlux imaging flow cytometer leverages the advantages of microfluidics and precise flow control. The CytoFlux system operates by precisely controlling the flow of a cellular suspension through a viewing region. The flow of the suspension is stopped many times per second to allow high resolution images to be captured. Imaging modalities can include brightfield, multiple fluorescent wavelengths, confocal, etc. The CytoFlux system is designed to mount to standard microscope stages.

Tunable Exposure Times

Allows low intensity fluorescent signals longer integration times for high quality images.

Self-Contained Disposable Fluidics

All fluidics are contained within a 96 well plate, eliminating the possibility of contamination or carryover.

Precise Focus, Improved Resolution

System fluidics retain precise focus at the same Z height for all cells imaged.

Broad Wavelength Capability

The CytoFlux system uses a simplified illumination system that imposes virtually no wavelength restrictions.

Download the CytoFlux System Specifications



Product Downloads CytoFlux Product Specifications CytoFlux Technology White Paper CytoFlux System Includes: Microfluidic Interface Control Module CytoFlux Software Tubing and Accessories	CytoFlux™ Imaging Flow Cytometer The CytoFlux imaging cytometer combines the statistical power and throughput of flow cytometry with the information-rich imaging of high resolution microscopy. The availability of individual cell images creates information-rich data on intracellular organization and cell morphology, thus enabling new applications that rely on intracellular imaging. The CytoFlux imaging flow cytometer leverages the advantages of microfluidics and precise flow control. The CytoFlux system operates by precisely controlling the flow of a cellular suspension through a viewing region. The flow of the suspension is stopped many times per second to allow high resolution images to be captured. Imaging modalities can include brightfield, multiple fluorescent wavelengths, confocal, etc. The CytoFlux system is designed to mount to standard microscope stages. Tunable Exposure Times Allows low intensity fluorescent signals longer integration times for high quality images. Self-Contained Disposable Fluidics All fluidics are contained within a 96 well plate, eliminating the possibility of contamination or carryover. Precise Focus, Improved Resolution System fluidics retain precise focus at the same Z height for all cells imaged. Broad Wavelength Capability The CytoFlux system uses a simplified illumination system that imposes virtually no wavelength restrictions. Download the CytoFlux System Specifications	Sample images during stopped flow. A number of fluorescently labeled cells are placed in the viewing window and images acquired in phase mode (A), fluorescence (B). Data containing single cell images is assembled in (C).
		Typical Applications
		Nuclear Factor kappa-B translocation Leukocyte classification Cell cycle studies Co-localization Cell-cell interactions CD4/CD8 studies Nuclear translocation Modes of apoptosis Intracellular trafficking


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