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At Fluxion, we’re passionate about delivering cell-based and cell-free solutions that facilitate the transformation of research discoveries into new ways to diagnose and treat patients. By characterizing molecular and cellular mechanisms of disease, Fluxion’s platforms help bridge the translational medicine gap, enabling rapid advances in disease research, drug discovery, and the development of diagnostic tests.

Cellular Analysis

Montage Cell Analysis Software

 

Automated software for advanced experimental control and data analysis

Live cell imaging requires a sophisticated array of hardware devices to produce high quality image data. Managing this can be tricky, but BioFlux Montage software provides a single-point solution to make your live-cell experiments easier to control than ever before. BioFlux Montage takes control of all microscopy hardware and image acquisition devices to produce high throughput, automated data from your BioFlux experiments. When it comes time for analysis, a comprehensive suite of analysis tools makes the process fast, simple, and powerful.

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Application Modules

Several application modules are available to add to the base configuration of BioFlux Montage. These modules are dedicated to specific routines which are common to live cell imaging. Extensive controls are provided to tailor the analysis to the application requirements. Once parameters are defined, these modules can automatically process large data sets in an automated, unattended manner. Data is readily usable in tabular or graphical formats.

Used for assessing cell viability and is compatible with commercially available Live/Dead assay kits designed to study cell proliferation or death. Common applications monitor cell proliferation associated with cancer or premature cell death involved in neuromuscular diseases such as Alzheimer's and Parkinson's, in addition to cytotoxicity and apoptotic events. Probes can target any part of cell and do not require a nuclear marker.

Tracks movement of individual cells or other cell related objects as they move across time-lapse image series. Other common measurements such as object velocity, angular trajectory, spatial distance, distance from origin can then be derived from this information. Useful for leukocyte rolling, cell motility, chemotaxis, and cell migration studies.

Enables scoring of individual cells for multi-parametric analyses. Cells are first identified and counted automatically using a nuclear stain. Other fluorescent markers can then be scored to provide population statistics and place the staining results within a specific context. Useful for cytotoxicity and gene expression profiling. Optimize settings for up to 7 probes (including nuclear probe) to customize analysis of 7 markers.

Designed to measure and characterize outgrowths (both length and branching), the extension of axonal processes from the cell body, both of which are natural parts of neuronal development. This module is also useful for evaluating neurotoxicity of compounds intended for other cellular targets. Unique assay that cannot be performed without imaging. Provides consistent results faster than manual tracing and counting.

Software Packages

BioFlux Montage

A complete software solution

BioFlux Montage enables full hardware control, image acquisition, and data analysis. It features the BioFlux Control Module to synchronize BioFlux experiments with imaging data acquisition. Optional data analysis modules are available to provide application-specific solutions with easy to use interfaces.

BioFlux Offline

A flexible addition to your BioFlux system

BioFlux Montage Offline is a process-only software package which performs the full suite of Montage data analysis. This version can be used offline from the BioFlux 1000z system to enable remote use of the data processing capabilities. BioFlux Montage Offline can also complement a BioFlux 200 system by adding a complete image analysis solution.

 

Key Features

  • Automated image acquisition: Image acquisition is the core capability of the BioFlux 1000z workstation. BioFlux Montage controls all aspects of this process including camera and time lapse settings. It easily manages and recalls illumination settings in brightfield, phase, and fluorescence applications. Camera controls include gain, regions of interest, and binning. Time lapse settings ensure the proper acquisition rate for extended duration experiments. All images are synchronized to BioFlux flow protocols and tagged with the relevant settings.

  • Automate BioFlux routines from a single control point: BioFlux Montage provides a simple, intuitive platform for controlling BioFlux experiments. The BioFlux Control Module is integrated with image acquisition and hardware control for complete access to all data acquisition parameters. Images are synchronized and labeled with BioFlux flow properties to keep track of the physiological conditions.

  • Advanced hardware control: BioFlux Montage controls all essential hardware components of the BioFlux 1000z automated microscopy platform. This includes advanced control over the motorized scanning stage, fluorescence filter wheels and shutters, objective nosepiece, and auto-focus hardware. The software provides digital algorithms for auto-focus to ensure clean, crisp images over time lapse acquisitions.

For More Information

→ Download the BioFlux Montage Brochure

If you have any additional questions please contact our product specialists.

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