Join Dr. Lara Toffali from the University of Verona for a Webinar about BeQuanti and the BioFlux System
A key function of the immune system is the regulation of leukocyte extravasation, the very first step of any immune response. Mandatory to study this homeostatic process is the capability of analyzing cell adhesion under flow conditions, thus reconstructing, in vitro, the in vivo behavior of cells interacting under wall shear stress with the endothelial cells in micro vessels. Different methodologies have been developed in the past two decades to perform this kind of experiments, such as, for instance, the Fluxion BioFlux system, all implying recording of digital movies propaedeutic to frame-by-frame analysis of cell behavior. However, highly trained personnel are needed to perform movie analysis and, although time consuming, tedious, inaccurate and prone to errors, human operated analysis is still the standard approach in the community. To definitively overcome these limitations, we have developed BeQuanti, innovative software allowing automatic cell recognition and flexible, yet very accurate, quantitative multi parametric analysis of cell adhesion events under flow. We describe the implementation and the main features of the software and also provide case studies in different experimental contexts, showing that BeQuanti is a very innovative bioinformatics tool allowing unique and powerful analysis of flow cell adhesion experiments, characterized by an efficiency, speed and accuracy impossible to obtain manually.