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At Fluxion, we’re passionate about delivering cell-based and cell-free solutions that facilitate the transformation of research discoveries into new ways to diagnose and treat patients. By characterizing molecular and cellular mechanisms of disease, Fluxion’s platforms help bridge the translational medicine gap, enabling rapid advances in disease research, drug discovery, and the development of diagnostic tests.

Sickle Cell Disease

and preclinical drug validation

Since the early 1900s, there has only been one FDA-approved therapy for the prevention of SCD-related complications. Fortunately, this is an historic moment in sickle cell discovery, with the largest pipeline of promising SCD-specific drugs in preclinical and clinical trials in the history of the disease. In addition to targeting hemoglobin polymerization, many of these drugs target the unique erythrocyte adhesive properties that contribute to microvascular obstruction.  Despite this, there is no clinical standard for assessing erythrocyte adhesion. SCD providers will soon have drugs available to prevent and relieve adhesion-mediated complications, therefore there is an urgent need diagnostic tools to guide drug selection for individual patients and monitor therapeutic response.

By using BioFlux, flow adhesion assays for SCD are made easy. The system can be utilized to understand the adhesive properties of sickle erythrocytes in a simulated blood flow environment, and the process is currently being worked on to be standardize for clinical testing. In fact, a US company (Functional Fluidics) has deployed a standardized microfluidic flow-based test into the preclinical and clinical research market. The advances in microfluidic flow-based blood function testing with BioFlux play a crucial role in preclinical drug validation, the potential for applications in sickle cell clinical trials, and ultimately clinical therapy. 

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Functionalized BioFlux flow channels were used to analyze eryptotic erythrocytes adhering to VWF fibers (Nicolay et al., 2018).

 

Microfluidic Flow-Based Diagnostics Used to Personalize SCD Therapy

Webinar discussing advances in microfluidic flow-based blood function testing, the role these tests are play in preclinical drug validation, and the potential for applications in sickle cell clinical trials and ultimately clinical therapy.

BioFlux Webinar - Can microfluidic flow-based diagnostics personalize therapy in sickle cell disease

 

Key BioFlux Advantages For SCD Studies

  • Utilize pulsatile flow function, where the shear force can oscillate from 0 to 200 dyne/cm² when using the high shear well plate

  • Analyze shear-dependent cellular responses, such as sickle erythrocyte (SSBRC) adhesion to the endothelium

  • Test for novel anti-adhesion compounds in a high throughput context

  • Time to perform an entire adhesion experiment from plate coating to data collection is reduced to 3 hours (in comparison to 6-18 hours for a standard microplate adhesion inhibition assay)

Core Technology

BioFlux System

Cellular Analysis
Core Technology

BioFlux System

Cellular Analysis
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Discovery Services

Sickle Cell Disease Assays

Optimized Lab Services
Discovery Services

Sickle Cell Disease Assays

Optimized Lab Services
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Application Notes

Publications & Posters

Webinars

Genetic Control of Antifungal Resistance in Candida albicans

Presented: 2020-02-28

Speaker: Craig Ennis, University of...

Modeling catheter-blocking biofilms using the BioFlux system

Presented: 2019-12-05

Speaker: Nathan Blood, University of...

BioFlux Update on All Silicone Bottom Plates, Custom Bottom Plates and Dual Gas Flow

Presented: 2018-05-14

Speaker: Dr. Bryan Haines, PhD,...

Oral Biofilms Studies Using BioFlux Technology

Presented: 2016-10-25

Speaker: Bryan Haines, PhD, Fluxion...

Studying Fungus-Bacterium Interactions Under Dynamic Conditions

Presented: 2015-02-05

Speaker: Dr. Bastiaan P. Krom Ph.D.,...