and cardiovascular disease
It is well known that shear stress from fluid flow can have a profound impact on cellular behavior. Particularly in vascular biology, the effect of blood flow cannot be overlooked when assessing pharmacological response or the underlying mechanisms of the vasculature. In the presence of cardiovascular disease, where blood vessels can narrow almost to occlusion, the shear stress seen by the vessel wall amplifies considerably. This shear stress level significantly alters platelet and endothelial cell morphology and receptor expression.
BioFlux provides a well-controlled platform for studying thrombosis in these different shear conditions.
Whole blood with hematoporphyrin flowed through BioFlux microfluidic channels lined with endothelial cells. A microscope light was used to photochemically injure the the endothelium (A). Platelet adhesion and PF4 activation was studied in the channels. (B). Top-down (C) and sagittal (D) confocal images were taken of the endothelial damage (Poncz et al., 2017).
(A) Thrombus formation on collagen at different shear rates and time points. (B) Number of adhered platelets over time (Xi et al., 2016).
Key BioFlux Advantages For Thrombosis Studies
Explore of the differences in thrombus formation from donors with different or mutant genotypes
Discover and pre-clinically assess compounds for both anti-platelet and anti-thrombotic compounds
Characterize thrombosis pathways using knockout animals
Analyze the multiple biochemical interactions that become important for supporting platelet-surface interactions leading to thrombus formation