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At Fluxion, we’re passionate about delivering cell-based and cell-free solutions that facilitate the transformation of research discoveries into new ways to diagnose and treat patients. By characterizing molecular and cellular mechanisms of disease, Fluxion’s platforms help bridge the translational medicine gap, enabling rapid advances in disease research, drug discovery, and the development of diagnostic tests.

Leukocyte Adhesion & Rolling

Visualization of cellular processes in real-time provides invaluable insight into cell biology. Cell adhesion through protein-protein or protein-carbohydrate interactions plays an important role indirecting cell migration, gene expression, cell growth, morphological changes during development, and programmed cell death.

The BioFlux system is particularly adaptable to cell adhesion & rolling screens and can be performed under physiologically-relevant shear flow conditions. With this system, cell rolling, cell adhesion, and adhesion inhibition can be quantitated under continuous flow, providing major work flow advantages over traditional adhesion assays.

cell+adhesion+rolling

Functionalized BioFlux flow channels were used to analyze eryptotic erythrocytes adhering to VWF fibers (Nicolay et al., 2018).

 

Jurkat cells rolling on E-selectin

Jurkat T cells rolling over E-selectin at 2 dyn.

BioFlux System_ Cell Rolling

 

Key BioFlux Advantages For Cell Adhesion & Rolling Studies

  • Study live cell adhesion and rolling in a physiologically-relevant assay using primary endothelial cells, human peripheral blood mononuclear cells, and more

  • Utilize time-lapse microscopy under flow and post-experiment image analysis to determine rolling distance

  • Examine dozens of different monolayers at one time in order to determine IC50 concentrations or screen multiple compounds under the same experimental conditions

  • Calculate area of platelet adhesion by utilizing BioFlux software analysis capabilities

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