Leukocyte Adhesion & Rolling
Visualization of cellular processes in real-time provides invaluable insight into cell biology. Cell adhesion through protein-protein or protein-carbohydrate interactions plays an important role indirecting cell migration, gene expression, cell growth, morphological changes during development, and programmed cell death.
The BioFlux system is particularly adaptable to cell adhesion & rolling screens and can be performed under physiologically-relevant shear flow conditions. With this system, cell rolling, cell adhesion, and adhesion inhibition can be quantitated under continuous flow, providing major work flow advantages over traditional adhesion assays.
Functionalized BioFlux flow channels were used to analyze eryptotic erythrocytes adhering to VWF fibers (Nicolay et al., 2018).
Jurkat cells rolling on E-selectin
Jurkat T cells rolling over E-selectin at 2 dyn.
Key BioFlux Advantages For Cell Adhesion & Rolling Studies
Study live cell adhesion and rolling in a physiologically-relevant assay using primary endothelial cells, human peripheral blood mononuclear cells, and more
Utilize time-lapse microscopy under flow and post-experiment image analysis to determine rolling distance
Examine dozens of different monolayers at one time in order to determine IC50 concentrations or screen multiple compounds under the same experimental conditions
Calculate area of platelet adhesion by utilizing BioFlux software analysis capabilities