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At Fluxion, we’re passionate about delivering cell-based and cell-free solutions that facilitate the transformation of research discoveries into new ways to diagnose and treat patients. By characterizing molecular and cellular mechanisms of disease, Fluxion’s platforms help bridge the translational medicine gap, enabling rapid advances in disease research, drug discovery, and the development of diagnostic tests.

Platelet Aggregation & Adhesion

and vascular injury

Visualization of cellular processes in real-time provides invaluable insight into cell biology. Cell adhesion through protein-protein or protein-carbohydrate interactions plays an important role indirecting cell migration, gene expression, cell growth, morphological changes during development, and programmed cell death.

The BioFlux system is particularly adaptable to platelet adhesion & aggregation screens and can be performed under physiologically-relevant shear flow conditions. With this system, platelet rolling, platelet adhesion, platelet aggregation, and adhesion inhibition can be quantitated under continuous flow, providing major work flow advantages over traditional adhesion assays.

platlet adhesion

Functionalized BioFlux flow channels were used to analyze the effect of guanosine on platelet adhesion and aggregation, mimicking arterial flow conditions. Guanosine was shown to inhibit platelet aggregation (Palomo et al., 2013).

 

Calcein AM Labeled Platelet Aggregation on Type I collagen

Whole blood labeled with calcein AM flowing over collagen 1 at 40 dyn over 5 minutes.

BioFlux System_ CalceinAM Labeled Platelet Aggregation on Collagen 1

 

Key BioFlux Advantages For Platelet Aggregation & Adhesion Studies

  • Study live platelet adhesion and aggregation in a physiologically-relevant assay using primary endothelial cells, human peripheral blood mononuclear cells, and more

  • Investigation of kinetic platelet adhesion and aggregation behavior on different substrates over many parallel conditions

  • Utilize time-lapse microscopy under flow and post-experiment image analysis to determine rolling distance

  • Examine dozens of different monolayers at one time in order to determine IC50 concentrations or screen multiple compounds under the same experimental conditions

  • Calculate area of platelet adhesion by utilizing BioFlux software analysis capabilities

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