and autoimmune disease
Inflammation plays a large role in rheumatic diseases, whether it be gout, lupus, or rheumatoid arthritis (RA). With systemic autoimmune rheumatic diseases (SARD), white blood cells mistakenly attack the body’s own tissues, leading to inflammation. In order to fully understand inflammation in these circumstances, each step of the process needs to be analyzed in a controlled environment.
The BioFlux system is an easy to use, automated platform for investigation of rheumatic disease under shear flow and high throughput. Immune cell rolling, adhesion, aggregation, and activation can all be studied with BioFlux. Analyzing these processes under shear flow is crucial to maintain physiological relevance and mimic behavior in the vasculature.
Visualization of the BioFlux chamber. Osteoblasts grew and adhered to the BioFlux microfluidic well plate, then were subjected to shear stress in order to study the precursors of bone mass reduction in vivo (Noda et al., 2013).
(A) Anticoagulated blood was labeled with calcien AM and incubated with different concentrations of GS-9876, and perfused through collagen-coated channels at 10 dynes/cm^2. (B) GS-9876 was shown to block platelet adhesion and aggregation (Di Paolo et al., 2018).
Key BioFlux Advantages For Rheumatology Studies
Study live immune cell adhesion and rolling in a physiologically-relevant assay
Utilize time-lapse microscopy under flow and post-experiment image analysis to determine immune cell rolling distance
Calculate area of immune cell adhesion by utilizing BioFlux software analysis capabilities
Investigation of platelet and neutrophil adhesion and aggregation on different substrates over many parallel conditions