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At Fluxion, we’re passionate about delivering cell-based and cell-free solutions that facilitate the transformation of research discoveries into new ways to diagnose and treat patients. By characterizing molecular and cellular mechanisms of disease, Fluxion’s platforms help bridge the translational medicine gap, enabling rapid advances in disease research, drug discovery, and the development of diagnostic tests.

Potassium Channels

Voltage-gated potassium channels, specially KV 11.1 (hERG), have long been the main target for cardiac safety pharmacology. hERG K+ channel inhibition by both cardiac and non-cardiac drugs have been identified as the most common cause of acquired, drug-induced LQTS (Long QT Syndrome) that may lead to sudden cardiac death. In fact, the side effect of hERG K+ channel inhibition is one of the major reasons of drug withdrawal or drug re-labeling. The gold standard of evaluating drug effects on hERG K+ current is manual patch clamp recording. However, this low-throughput, high-cost approach is limiting in safety screening of large numbers of drugs. Recently, automated electrophysiology systems have been developed that can obtain high-throughput recordings and achieve reasonably comparable results with manual patch clamp. However a major limitation started to appear in many HTS systems studying hERG and potassium channels in general is the lack of assay stability and longevity compared to manual patch clamp studies.

IonFlux Mercury complements manual patch clamp systems by delivering plate reader simplicity to complicated ion channel assays. IonFlux Mercury features include:

  • Continuously flowing solutions prolong assay stability

  • Short additions followed by controlled continuous wash of drugs allow calculation of binding kinetics

  • Precise temperature control allows physiological condition for drug interactions

herg+IV

hERG tail current affected by increasing voltage relationship

 

Inward Cl- current from one ensemble of cells exposed to GABA with increasing concentrations (1μM to 100μM)

Rapid change of solutions during GABA current activation sweeps.

Core Technology

IonFlux Mercury

Automated Patch Clamp System
Core Technology

IonFlux Mercury

Automated Patch Clamp System
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IonFlux Mercury
Cell Lines

Potassium Channels

Validated Stable Cell lines
Cell Lines

Potassium Channels

Validated Stable Cell lines
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Cell Culture
Discovery Services

Potassium Channels Assays

Optimized Lab Services
Discovery Services

Potassium Channels Assays

Optimized Lab Services
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Discovery Services

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