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At Fluxion, we’re passionate about delivering cell-based and cell-free solutions that facilitate the transformation of research discoveries into new ways to diagnose and treat patients. By characterizing molecular and cellular mechanisms of disease, Fluxion’s platforms help bridge the translational medicine gap, enabling rapid advances in disease research, drug discovery, and the development of diagnostic tests.

Liquid Biopsy

IsoFlux CTC & Rare Cell Anlysis Kits

IsoFlux targeted enrichment delivers the highest sensitivity and specificity

The IsoFlux Instrument utilizes a number of different kits for isolation of CTCs and other rare cell types from biological samples (whole blood, frac0onated blood, ascites, etc.). Each kit incorporates eight (8) IsoFlux microfluidic cartridges for high-sensitivity immunomagnetic enrichment and the reagents necessary to perform positive cell enrichment for eight samples. Available antibodies include both epithelial and mesenchymal markers, depending on the desired cell type. The kits described are intended for use with IsoFlux instrument. For research use only.

Kit Description
Enhanced CTC Kit, 8 pack (EpCAM/EGFR)
Validated for use with the enumeration kit and the IsoFlux CTC NGS Prep Kit. Recovery based on EpCAM and EGFR. The most commonly used CTC enrichment kit.
CTC Enrichment Kit, 8 pack (EpCAM)
Validated for high sensitivity recovery of CTCs. Recovery based on EpCAM antigen presence. Validated for use with the IsoFlux Enumeration Kit and the the CTC NGS Prep Kit.
EMT Enrichment Kit, 8 pack (EpCAM/EGFR/Mesenchymal)
Validated to recover both epithelial and mesenchymal cell types, this is the most comprehensive set of recovery markers.  Five different markers are used, including three mesenchymal markers, EpCAM, and EGFR. Validated for use with the CTC NGS Prep Kit. 
Rare Cell Enrichment Kit, 8 Pack (User-customized)
The RCE kit was designed with maximum flexibility in mind. The magnetic bead surface is coated with pan-mouse IgG molecules, allowing users to couple their own antibodies to the beads for targeted cell capture.
IsoFlux Enumeration Kit
Reagent and antibody kit for enumeration of CTCs in IsoFlux samples using immunofluorescence. CTCs are defined as CK+, CD45-, and nucleated intact cells. Contains enough reagent to stain 200 IsoFlux samples or more depending on usage habits. 
IsoFlux CTC NGS Prep Kit

Designed for processing of IsoFlux CTC samples, the NGS Prep Kit prepares the recovered CTCs for genomic analysis. The kit contains purity enhancement columns and WGA reagents, producing DNA ready for NGS library prep or PCR. Used in conjunction with Fluxion’s Spotlight Oncology Panels, the NGS Prep Kit creates a complete sample-to-answer assay for high-sensitivity mutation detection of solid tumor cancers from CTCs. 

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If you have any questions or would like to request a quote, please contact our product specialists.

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