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At Fluxion, we’re passionate about delivering cell-based and cell-free solutions that facilitate the transformation of research discoveries into new ways to diagnose and treat patients. By characterizing molecular and cellular mechanisms of disease, Fluxion’s platforms help bridge the translational medicine gap, enabling rapid advances in disease research, drug discovery, and the development of diagnostic tests.

Engineered T-Cell

Immunotherapies

BioFlux is ideal for engineered T cell functional testing

Immunotherapies such as CAR-T and TCR function by reaching their targets through a complex process that can involve interactions between multiple immune cell types and the target tissue, as well as interactions between the immune cells themselves.

Modeling of this process, and ensuring that the engineered T cells have the desired functional properties, requires an in vitro model that can effectively mimic the physiological conditions in the human body. To achieve this, Fluxion has developed the BioFlux "artery on a chip" systems that serve as effective platforms for these functional cell assays. These assays are designed to assess the cell trafficking process, including homing, adhesion, and transmigration, and serve as the ideal platform for functional testing of engineered T cell therapies such as CAR-T and TCR.

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Treated Jurkat cells are showing adhered to an endothelial cell monolayer. Experiments can be run as time lapse or endpoint, and data includes degree of cell adherence and transmigration relative to controls. 

 

KEY BIOFLUX ADVANTAGES FOR ENGINEERED T CELL DEVELOPMENT

  • Study live cell transmigration in a physiologically-relevant assay using primary endothelial cells, cancer cells, or protein layers
  • Run the assay as time lapse to capture the process of adhesion and transmigration, or run as endpoint for high throughput discovery
  • Rapidly determine IC50 concentrations of multiple compounds under the same experimental conditions
  • Calculate number of adhered and transmigrated cells automatically utilizing BioFlux software analysis capabilities

Application Notes

Publications

Webinars

Sialic acid and galectins mediate xenogeneic neutrophil-endothelial adhesion

Speakers: Dr. Beth French, University of Maryland

Abstract:

Using BioFlux to Study Cell Rolling

Speaker: Bryan Haines, PhD, Fluxion Biosciences

Dr. Bryan...

Quantitation of Cell Migration using the BioFlux System

Speaker: Josephine Nalbantoglu, PhD, Associate Professor,...

BeQuanti: software for automatic detection and analysis of cell adhesion under flow

Speaker: Lara Toffali, University of Verona

BeQuanti:...

Applications and Opportunities for Using the 24-Well Dual Flow Plates

Speaker: Bryan Haines, PhD, Fluxion Biosciences

BioFlux...

Biomechanopharmacological study of traditional Chinese medicine

Speakers: Dr. You Yun and Dr. Liao Fulong, China Academy of...

Effects of shear stress on uterine vasculature remodeling by trophoblasts

Speaker: Dr. Jo James, University of Auckland

Abstract:

...

Enhance your platelet assays with flow

Speaker: Carolyn Conant, PhD, Fluxion Biosciences

Abstract:

See What You’ve Been Missing in Your Cell Based Assays

Speaker: Carolyn Conant PhD, Fluxion Biosciences

...

Capturing Dynamic Data from Migration, Invasion, and Angiogenesis Assays

Speaker: Carolyn Connant, PhD, Fluxion Biosciences

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