and cancer treatment
Tumors have a close connection to the environment surrounding them. From lymphocytes to signaling molecules, tumors interact with and strongly influence their microenvironment. Tumors can promote immunotolerance, encourage tumor angiogenesis, and create several other issues for the surrounding area. In order to comprehend these intricacies in full, analyzing the microenvironment in vitro is crucial.
The BioFlux system is a high-throughput microfluidic platform ideal for analysis of several oncological processes, including the development and behavior of a tumor microenvironment. By controlling for pressure, temperature, and flow, BioFlux creates a physiologically relevant atmosphere for an oncological assay.
MCF-7 cells (alone and LPS-treated THP-1) adherent to HUVEC layers under shear flow (Khismatullin et al., 2014).
A BioFlux system was used to analyze CHO cell binding on prolectin/streptavidin coated channels at a shear stress levels under 0.1 dyn/cm². Prolectin binding and expression was studied in several different circumstances under shear flow in BioFlux channels (for more information, see Le Pendu et al., 2016).
Key BioFlux Advantages For Tumor Microenvironment Studies
Create a hypoxic environment, mimicking tumor microenvironment, with BioFlux’s gas control option
Manipulate microenvironment by flowing anti-inflammatory compounds or anti-cancer treatments into channels
Closely examine the connections between tumor and surrounding cells or molecules on a microfluidic platform
Pinpoint each aspect of microenvironment in a high throughput context, taking advantage of up to 24 experiments per plate
Couple BioFlux analysis with IsoFlux CTC isolation technology