Contact Us

At Fluxion, we’re passionate about delivering cell-based and cell-free solutions that facilitate the transformation of research discoveries into new ways to diagnose and treat patients. By characterizing molecular and cellular mechanisms of disease, Fluxion’s platforms help bridge the translational medicine gap, enabling rapid advances in disease research, drug discovery, and the development of diagnostic tests.

Stem Cell

Research

Visualization of stem cell interactions under flow helps elucidate stem cell recruitment, differentiation, migration. Stem cells are increasingly studied as potential sources of therapeutic treatment, and the study of these processes under physiological conditions is critical.

The BioFlux system is particularly use for for stem cell studies under physiologically-relevant shear flow conditions. With this system, recruitment, cell adhesion, and transmigration can be quantitated under continuous flow, providing major work flow advantages over traditional flow cell assays.

 

Below is an example of a stem cell study using BioFlux

stem cells BioFlux

hPMVECs were seeded in BioFlux microfluidic channels to form monolayers and the application of 2 dyn/cm2 pressure allowed fluorescently labelled MSCs to pass over the monolayer under shear flow. Still images from recordings indicate little to no binding of MSCs (A) compared to Mo control cells under the same conditions (B). hPMVEC monolayers were treated with PBS (C, Panel ii) or LPS (C, Panel iii) before MSC suspensions were passed over the layers. Flow was stopped and the cells were allowed to settle on the monolayer for 30minutes (C, Panel i) before re-initiation of the flow. Once all cells had passed through the chamber, images were acquired of the remaining, firmly adherent cells (C, Panels i, ii). MSC: Mesenchymal Stem Cell; LPS: Lipopolysaccharide; Mo: Monocyte; hPMVEC: human pulmonary microvascular endothelial cells.

Key BioFlux Advantages For STEM Cell research

  • Accurately model the in vivo environment: study stem cell interaction and transmigration to cell layers such as HUVECs

  • Generate superior data: utilize time-lapse microscopy under flow and post-experiment image analysis to determine degree of adhesion and transmigration

  • Get results fast: examine dozens of different treatments to see the effect on stem cell recruitment

  • Easily analyze data: calculate cell numbers adhered/transmigrated by utilizing BioFlux software analysis capabilities

BioFlux Lab Services

BioFlux Lab Services

Your assay,  our technology. immediate results

More Information
Lab Services

Knowledge Base

Knowledge Base

Get the latest guides, tutorials, videos and training documents for your BioFlux system

Access to Knowledge Base
Knwledge Base

Resources Library

Resources Library

Access to BioFlux publications, webinars and live demos

Resources Library
Close up of hand with laptop and media icons

For More Information about BioFlux Systems

Contact Us

 

 

CleanShot 2022-05-05 at 17-32-45@2x-1-1